Mouse Anti-AKT, FITC conjugated
Quantity size : 100ug (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. Reconstitute with 100ul sterile distilled water. )
background:
The serine-threonine protein kinase encoded by the AKT gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by plaet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Specificity : · anti- AKT is a mouse monoclonal antibody, FITC conjugated
· specific for anti- AKT /FITC of rat ,human and mouse.
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt：56kDa
Application : · FCM: 1:50-200
·IF : 1:50-1:200
· Immunocytochemistry: 1:50-1:200
· Excitation spectrum: 540 nm
· Emission spectrum: 625 nm
· Optimal working dilutions must be determined by the end user.
· protect from light.
Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 oC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
